Cell Image Analysis Technology Applications
Wound Healing Assays
| The wound healing assay in vitro is widely used for research and discovery in biology and medicine. This assay allows for observing the healing process in vitro in which the cells on the edges of the artificial wound migrate toward
the wound area. The influence of different culture conditions can be
measured by observing the change in the size of the wound area. For further investigation, more detailed measurements of the cell behaviors are required. We present an application of automatic cell tracking in phase-contrast microscopy images to wound healing assay.
The cell behaviors under three different culture conditions have been analyzed. Our cell tracking system can track individual cells during the healing process and provide detailed spatio-temporal measurements of cell behaviors including cell density, cell migration
speed and direction, and the statistics of cell mitosis events. The application demonstrates the effectiveness of automatic cell tracking for quantitative and detailed analysis of the cell behaviors in wound healing assay in vitro. |
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| Examples of cell behavior measurements | ||
| Cell Density | Cell Migration Direction | Cell Migration Speed |
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| Cell density changes over time, each row of which represents the density. | Cell migration paths (left and right groups) | The average speed of the cell migration at each local area. |
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| Distributions of cell migration directions in response to different culturing conditions. |